Adeno-associated virus (AAV)-mediated gene therapy has emerged as a highly promising and efficacious therapeutic over the last decade. Despite the versatility, relative safety and popularity of AAV, several challenges remain that still impede its mainstream use in the clinic, one of it being unavailability of detailed pharmacy recommendations. Pharmacists at Nationwide Children’s Hospital have developed a pharmacy training manual that describes in-depth pharmacy procedures and expert opinions related to the handling and manipulation of viral mediated gene therapy. This document would allow the harmonization of pharmacy procedures between multiple sites in a clinical trial.