TS-002315 — The standard human embryonic kidney 293 (HEK293) cell line is widely used in cancer research, vaccine production, adenovirus and adeno-associated viral vectors and gene therapy. Researchers at Nationwide Children’s Hospital have discovered a new use for the HEK293 cell line: generating stable HEK293 cells expressing MiB1-FLAG and hemagglutinin (HA)–ubiquitin. MiB1-FLAG plays a vital role in cell migration and ubiquitin marks HA for degradation and affects its activity. As a result, the HEK293 cells establish a proper immune response, wound repair and tissue homeostasis while attacking hemagglutinin?????

TS-001035 — Shiny R is an open source platform that allows a framework to develop online applications. With minimal required background in coding principles, a team of researchers at Nationwide Children’s Hospital were able to display and interact with the analysis made of single-cell transcriptome. Shiny R generates visualizations that include UMAP plots and presents features of single-cell RNA and transcriptomic data without extensive training in R programming. Improvements made on this existing technology includes importing data, cell filtration, principle component analysis, clustering, dimensional reduction, merging datasets, and Graphical user interface (GUI)-based generations of gene expression plots. This significantly improves the visualization and analysis of single-cell transcriptome analysis.