Physicians at Nationwide Children’s Hospital have developed a Tissue Engineered Vascular Graft (TEVG) by seeding patient cells onto a biodegradable tubular scaffold. The scaffold degrades by hydrolysis, ultimately leaving only the growing vessel in the patients. The Closed Seeding System enables efficient collection and seeding of patient cells onto the TEVG scaffold, which has been further optimized by using patient imaging data and 3D-printing capabilities to create patient-specific vascular grafts for implantation.