This novel mouse model is engineered to carry a human variant in ATP1A3, encoding the Na⁺/K⁺‑transporting ATPase alpha‑3 subunit. The model provides a unique in vivo platform for investigating the molecular and developmental mechanisms underlying ATP1A3‑associated neurodevelopmental disorders, serving as a valuable tool for disease‑mechanism discovery and proof‑of‑concept testing of potential therapeutic interventions.