Adeno-associated virus vectors (AAV) are used to deliver normal copies of genes or therapies to a targeted tissue or organ as treatment for many genetic disorders. Researchers at Nationwide Children’s Hospital created a process that will improve the production process for AAV viral vectors in flatware and bioreactors for suspension and adherent cells. They performed placid transfection prior to seeding into the tissue culture flasks which decreased the incubation time for cells in the bioreactor and yielded a higher crude AAV viral vector yield by 8-10-fold. Therefore, this process will reduce production time and costs of production.